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    Addgene inc work addgene id 219406 si
    Work Addgene Id 219406 Si, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/work+addgene+id+219406+si/pFlag-CMV2-Brd4+D1346N+(Plasmid+%2321940)/pm38980747__bm4c00346_si_001-23-289-290
    Average 92 stars, based on 1 article reviews
    work addgene id 219406 si - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Transformation Assay:

    Article Title: Protease-Driven Phase Separation of Elastin-Like Polypeptides.
    Article Snippet: E. coli Transformation Kit and Buffer Set Zymo Research Corporation Cat#T3002 Cloning Source Identifier NEBridge Golden Gate Assembly Kit (BsmBI-v2) New England Biolabs Cat#E1602S NEBridge Golden Gate Assembly Kit (BsaI-HF v2) New England Biolabs Cat#E1601S Plasmid-Safe ATP-Dependent DNAse LGC Biosearch Technologies Cat#NC9046399 SacI-HF New England Biolabs Cat#R3156S HindIII-HF New England Biolabs Cat#R3104S T4 DNA Ligase New England Biolabs Cat#M0202L Quick CIP New England Biolabs Cat#M0525S XhoI New England Biolabs Cat#R0146S NdeI New England Biolabs Cat#R0111S Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) New England Biolabs Cat#E0552S ZymoPURE Plasmid MiniPrep Kit Zymo Research Corporation Cat#50-125-1483 Zymoclean Gel DNA Recovery Kit Zymo Research Corporation Cat#11-301 Protein purification and validation Source Identifier Lysozyme, chicken egg white Sigma-Aldrich Cat#L6876-5G Ribonuclease A, bovine pancreas, chromatographically purified Thermo Fisher Scientific Cat#J62232EX3 DNase I Thermo Fisher Scientific Cat#EN0521 Fisher Science Education Seamless Cellulose Dialysis Tubing (14,000 MWCO) Thermo Fisher Scientific Cat#S25645H 12% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Laboratories Cat#4568046 Precision Plus Protein Unstained Standards Bio-Rad Laboratories Cat#1610363 Water Purification System Source Identifier Milli-Q Advantage A10 with BioPak Polisher, 18.2 MΩ·cm Millipore Sigma Cat# Z00Q0V0WW Proteases Source Identifier TEV Protease (TEVP) New England Biolabs Cat#P8112S Thrombin, Bovine, High Activity Calbiochem/Sigma-Aldrich Cat#604980-100U Factor Xa Protease New England Biolabs Cat#P8010S Enterokinase, light chain New England Biolabs Cat#P8070S Oligonucleotides Source Identifier ssDNA Integrated DNA Technologies N/A Gene fragments Source Identifier dsDNA Twist Bioscience N/A Recombinant DNA Source Identifier pGGAselect DNA New England Biolabs Component in Cat#E1602S and Cat#E1601S SI-3 Modified pET-22b(+) expression vector (SI section 4) This work - pET22b-Empty-cleavableELP (SI section 2) This work Addgene ID - 219401 pET22b-TEVP-cleavableELP This work Addgene ID - 219402 pET22b-FXa-cleavableELP This work Addgene ID - 219403 pET22b-EK-cleavableELP This work Addgene ID - 219404 pET22b-Thr-cleavableELP This work Addgene ID - 219405 pET22b-ELP[V]60 This work Addgene ID - 219406 SI-4 2.

    Cloning:

    Article Title: Protease-Driven Phase Separation of Elastin-Like Polypeptides.
    Article Snippet: E. coli Transformation Kit and Buffer Set Zymo Research Corporation Cat#T3002 Cloning Source Identifier NEBridge Golden Gate Assembly Kit (BsmBI-v2) New England Biolabs Cat#E1602S NEBridge Golden Gate Assembly Kit (BsaI-HF v2) New England Biolabs Cat#E1601S Plasmid-Safe ATP-Dependent DNAse LGC Biosearch Technologies Cat#NC9046399 SacI-HF New England Biolabs Cat#R3156S HindIII-HF New England Biolabs Cat#R3104S T4 DNA Ligase New England Biolabs Cat#M0202L Quick CIP New England Biolabs Cat#M0525S XhoI New England Biolabs Cat#R0146S NdeI New England Biolabs Cat#R0111S Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) New England Biolabs Cat#E0552S ZymoPURE Plasmid MiniPrep Kit Zymo Research Corporation Cat#50-125-1483 Zymoclean Gel DNA Recovery Kit Zymo Research Corporation Cat#11-301 Protein purification and validation Source Identifier Lysozyme, chicken egg white Sigma-Aldrich Cat#L6876-5G Ribonuclease A, bovine pancreas, chromatographically purified Thermo Fisher Scientific Cat#J62232EX3 DNase I Thermo Fisher Scientific Cat#EN0521 Fisher Science Education Seamless Cellulose Dialysis Tubing (14,000 MWCO) Thermo Fisher Scientific Cat#S25645H 12% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Laboratories Cat#4568046 Precision Plus Protein Unstained Standards Bio-Rad Laboratories Cat#1610363 Water Purification System Source Identifier Milli-Q Advantage A10 with BioPak Polisher, 18.2 MΩ·cm Millipore Sigma Cat# Z00Q0V0WW Proteases Source Identifier TEV Protease (TEVP) New England Biolabs Cat#P8112S Thrombin, Bovine, High Activity Calbiochem/Sigma-Aldrich Cat#604980-100U Factor Xa Protease New England Biolabs Cat#P8010S Enterokinase, light chain New England Biolabs Cat#P8070S Oligonucleotides Source Identifier ssDNA Integrated DNA Technologies N/A Gene fragments Source Identifier dsDNA Twist Bioscience N/A Recombinant DNA Source Identifier pGGAselect DNA New England Biolabs Component in Cat#E1602S and Cat#E1601S SI-3 Modified pET-22b(+) expression vector (SI section 4) This work - pET22b-Empty-cleavableELP (SI section 2) This work Addgene ID - 219401 pET22b-TEVP-cleavableELP This work Addgene ID - 219402 pET22b-FXa-cleavableELP This work Addgene ID - 219403 pET22b-EK-cleavableELP This work Addgene ID - 219404 pET22b-Thr-cleavableELP This work Addgene ID - 219405 pET22b-ELP[V]60 This work Addgene ID - 219406 SI-4 2.

    Plasmid Preparation:

    Article Title: Protease-Driven Phase Separation of Elastin-Like Polypeptides.
    Article Snippet: E. coli Transformation Kit and Buffer Set Zymo Research Corporation Cat#T3002 Cloning Source Identifier NEBridge Golden Gate Assembly Kit (BsmBI-v2) New England Biolabs Cat#E1602S NEBridge Golden Gate Assembly Kit (BsaI-HF v2) New England Biolabs Cat#E1601S Plasmid-Safe ATP-Dependent DNAse LGC Biosearch Technologies Cat#NC9046399 SacI-HF New England Biolabs Cat#R3156S HindIII-HF New England Biolabs Cat#R3104S T4 DNA Ligase New England Biolabs Cat#M0202L Quick CIP New England Biolabs Cat#M0525S XhoI New England Biolabs Cat#R0146S NdeI New England Biolabs Cat#R0111S Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) New England Biolabs Cat#E0552S ZymoPURE Plasmid MiniPrep Kit Zymo Research Corporation Cat#50-125-1483 Zymoclean Gel DNA Recovery Kit Zymo Research Corporation Cat#11-301 Protein purification and validation Source Identifier Lysozyme, chicken egg white Sigma-Aldrich Cat#L6876-5G Ribonuclease A, bovine pancreas, chromatographically purified Thermo Fisher Scientific Cat#J62232EX3 DNase I Thermo Fisher Scientific Cat#EN0521 Fisher Science Education Seamless Cellulose Dialysis Tubing (14,000 MWCO) Thermo Fisher Scientific Cat#S25645H 12% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Laboratories Cat#4568046 Precision Plus Protein Unstained Standards Bio-Rad Laboratories Cat#1610363 Water Purification System Source Identifier Milli-Q Advantage A10 with BioPak Polisher, 18.2 MΩ·cm Millipore Sigma Cat# Z00Q0V0WW Proteases Source Identifier TEV Protease (TEVP) New England Biolabs Cat#P8112S Thrombin, Bovine, High Activity Calbiochem/Sigma-Aldrich Cat#604980-100U Factor Xa Protease New England Biolabs Cat#P8010S Enterokinase, light chain New England Biolabs Cat#P8070S Oligonucleotides Source Identifier ssDNA Integrated DNA Technologies N/A Gene fragments Source Identifier dsDNA Twist Bioscience N/A Recombinant DNA Source Identifier pGGAselect DNA New England Biolabs Component in Cat#E1602S and Cat#E1601S SI-3 Modified pET-22b(+) expression vector (SI section 4) This work - pET22b-Empty-cleavableELP (SI section 2) This work Addgene ID - 219401 pET22b-TEVP-cleavableELP This work Addgene ID - 219402 pET22b-FXa-cleavableELP This work Addgene ID - 219403 pET22b-EK-cleavableELP This work Addgene ID - 219404 pET22b-Thr-cleavableELP This work Addgene ID - 219405 pET22b-ELP[V]60 This work Addgene ID - 219406 SI-4 2.

    Mutagenesis:

    Article Title: Protease-Driven Phase Separation of Elastin-Like Polypeptides.
    Article Snippet: E. coli Transformation Kit and Buffer Set Zymo Research Corporation Cat#T3002 Cloning Source Identifier NEBridge Golden Gate Assembly Kit (BsmBI-v2) New England Biolabs Cat#E1602S NEBridge Golden Gate Assembly Kit (BsaI-HF v2) New England Biolabs Cat#E1601S Plasmid-Safe ATP-Dependent DNAse LGC Biosearch Technologies Cat#NC9046399 SacI-HF New England Biolabs Cat#R3156S HindIII-HF New England Biolabs Cat#R3104S T4 DNA Ligase New England Biolabs Cat#M0202L Quick CIP New England Biolabs Cat#M0525S XhoI New England Biolabs Cat#R0146S NdeI New England Biolabs Cat#R0111S Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) New England Biolabs Cat#E0552S ZymoPURE Plasmid MiniPrep Kit Zymo Research Corporation Cat#50-125-1483 Zymoclean Gel DNA Recovery Kit Zymo Research Corporation Cat#11-301 Protein purification and validation Source Identifier Lysozyme, chicken egg white Sigma-Aldrich Cat#L6876-5G Ribonuclease A, bovine pancreas, chromatographically purified Thermo Fisher Scientific Cat#J62232EX3 DNase I Thermo Fisher Scientific Cat#EN0521 Fisher Science Education Seamless Cellulose Dialysis Tubing (14,000 MWCO) Thermo Fisher Scientific Cat#S25645H 12% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Laboratories Cat#4568046 Precision Plus Protein Unstained Standards Bio-Rad Laboratories Cat#1610363 Water Purification System Source Identifier Milli-Q Advantage A10 with BioPak Polisher, 18.2 MΩ·cm Millipore Sigma Cat# Z00Q0V0WW Proteases Source Identifier TEV Protease (TEVP) New England Biolabs Cat#P8112S Thrombin, Bovine, High Activity Calbiochem/Sigma-Aldrich Cat#604980-100U Factor Xa Protease New England Biolabs Cat#P8010S Enterokinase, light chain New England Biolabs Cat#P8070S Oligonucleotides Source Identifier ssDNA Integrated DNA Technologies N/A Gene fragments Source Identifier dsDNA Twist Bioscience N/A Recombinant DNA Source Identifier pGGAselect DNA New England Biolabs Component in Cat#E1602S and Cat#E1601S SI-3 Modified pET-22b(+) expression vector (SI section 4) This work - pET22b-Empty-cleavableELP (SI section 2) This work Addgene ID - 219401 pET22b-TEVP-cleavableELP This work Addgene ID - 219402 pET22b-FXa-cleavableELP This work Addgene ID - 219403 pET22b-EK-cleavableELP This work Addgene ID - 219404 pET22b-Thr-cleavableELP This work Addgene ID - 219405 pET22b-ELP[V]60 This work Addgene ID - 219406 SI-4 2.

    Protein Purification:

    Article Title: Protease-Driven Phase Separation of Elastin-Like Polypeptides.
    Article Snippet: E. coli Transformation Kit and Buffer Set Zymo Research Corporation Cat#T3002 Cloning Source Identifier NEBridge Golden Gate Assembly Kit (BsmBI-v2) New England Biolabs Cat#E1602S NEBridge Golden Gate Assembly Kit (BsaI-HF v2) New England Biolabs Cat#E1601S Plasmid-Safe ATP-Dependent DNAse LGC Biosearch Technologies Cat#NC9046399 SacI-HF New England Biolabs Cat#R3156S HindIII-HF New England Biolabs Cat#R3104S T4 DNA Ligase New England Biolabs Cat#M0202L Quick CIP New England Biolabs Cat#M0525S XhoI New England Biolabs Cat#R0146S NdeI New England Biolabs Cat#R0111S Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) New England Biolabs Cat#E0552S ZymoPURE Plasmid MiniPrep Kit Zymo Research Corporation Cat#50-125-1483 Zymoclean Gel DNA Recovery Kit Zymo Research Corporation Cat#11-301 Protein purification and validation Source Identifier Lysozyme, chicken egg white Sigma-Aldrich Cat#L6876-5G Ribonuclease A, bovine pancreas, chromatographically purified Thermo Fisher Scientific Cat#J62232EX3 DNase I Thermo Fisher Scientific Cat#EN0521 Fisher Science Education Seamless Cellulose Dialysis Tubing (14,000 MWCO) Thermo Fisher Scientific Cat#S25645H 12% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Laboratories Cat#4568046 Precision Plus Protein Unstained Standards Bio-Rad Laboratories Cat#1610363 Water Purification System Source Identifier Milli-Q Advantage A10 with BioPak Polisher, 18.2 MΩ·cm Millipore Sigma Cat# Z00Q0V0WW Proteases Source Identifier TEV Protease (TEVP) New England Biolabs Cat#P8112S Thrombin, Bovine, High Activity Calbiochem/Sigma-Aldrich Cat#604980-100U Factor Xa Protease New England Biolabs Cat#P8010S Enterokinase, light chain New England Biolabs Cat#P8070S Oligonucleotides Source Identifier ssDNA Integrated DNA Technologies N/A Gene fragments Source Identifier dsDNA Twist Bioscience N/A Recombinant DNA Source Identifier pGGAselect DNA New England Biolabs Component in Cat#E1602S and Cat#E1601S SI-3 Modified pET-22b(+) expression vector (SI section 4) This work - pET22b-Empty-cleavableELP (SI section 2) This work Addgene ID - 219401 pET22b-TEVP-cleavableELP This work Addgene ID - 219402 pET22b-FXa-cleavableELP This work Addgene ID - 219403 pET22b-EK-cleavableELP This work Addgene ID - 219404 pET22b-Thr-cleavableELP This work Addgene ID - 219405 pET22b-ELP[V]60 This work Addgene ID - 219406 SI-4 2.

    Biomarker Discovery:

    Article Title: Protease-Driven Phase Separation of Elastin-Like Polypeptides.
    Article Snippet: E. coli Transformation Kit and Buffer Set Zymo Research Corporation Cat#T3002 Cloning Source Identifier NEBridge Golden Gate Assembly Kit (BsmBI-v2) New England Biolabs Cat#E1602S NEBridge Golden Gate Assembly Kit (BsaI-HF v2) New England Biolabs Cat#E1601S Plasmid-Safe ATP-Dependent DNAse LGC Biosearch Technologies Cat#NC9046399 SacI-HF New England Biolabs Cat#R3156S HindIII-HF New England Biolabs Cat#R3104S T4 DNA Ligase New England Biolabs Cat#M0202L Quick CIP New England Biolabs Cat#M0525S XhoI New England Biolabs Cat#R0146S NdeI New England Biolabs Cat#R0111S Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) New England Biolabs Cat#E0552S ZymoPURE Plasmid MiniPrep Kit Zymo Research Corporation Cat#50-125-1483 Zymoclean Gel DNA Recovery Kit Zymo Research Corporation Cat#11-301 Protein purification and validation Source Identifier Lysozyme, chicken egg white Sigma-Aldrich Cat#L6876-5G Ribonuclease A, bovine pancreas, chromatographically purified Thermo Fisher Scientific Cat#J62232EX3 DNase I Thermo Fisher Scientific Cat#EN0521 Fisher Science Education Seamless Cellulose Dialysis Tubing (14,000 MWCO) Thermo Fisher Scientific Cat#S25645H 12% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Laboratories Cat#4568046 Precision Plus Protein Unstained Standards Bio-Rad Laboratories Cat#1610363 Water Purification System Source Identifier Milli-Q Advantage A10 with BioPak Polisher, 18.2 MΩ·cm Millipore Sigma Cat# Z00Q0V0WW Proteases Source Identifier TEV Protease (TEVP) New England Biolabs Cat#P8112S Thrombin, Bovine, High Activity Calbiochem/Sigma-Aldrich Cat#604980-100U Factor Xa Protease New England Biolabs Cat#P8010S Enterokinase, light chain New England Biolabs Cat#P8070S Oligonucleotides Source Identifier ssDNA Integrated DNA Technologies N/A Gene fragments Source Identifier dsDNA Twist Bioscience N/A Recombinant DNA Source Identifier pGGAselect DNA New England Biolabs Component in Cat#E1602S and Cat#E1601S SI-3 Modified pET-22b(+) expression vector (SI section 4) This work - pET22b-Empty-cleavableELP (SI section 2) This work Addgene ID - 219401 pET22b-TEVP-cleavableELP This work Addgene ID - 219402 pET22b-FXa-cleavableELP This work Addgene ID - 219403 pET22b-EK-cleavableELP This work Addgene ID - 219404 pET22b-Thr-cleavableELP This work Addgene ID - 219405 pET22b-ELP[V]60 This work Addgene ID - 219406 SI-4 2.

    Purification:

    Article Title: Protease-Driven Phase Separation of Elastin-Like Polypeptides.
    Article Snippet: E. coli Transformation Kit and Buffer Set Zymo Research Corporation Cat#T3002 Cloning Source Identifier NEBridge Golden Gate Assembly Kit (BsmBI-v2) New England Biolabs Cat#E1602S NEBridge Golden Gate Assembly Kit (BsaI-HF v2) New England Biolabs Cat#E1601S Plasmid-Safe ATP-Dependent DNAse LGC Biosearch Technologies Cat#NC9046399 SacI-HF New England Biolabs Cat#R3156S HindIII-HF New England Biolabs Cat#R3104S T4 DNA Ligase New England Biolabs Cat#M0202L Quick CIP New England Biolabs Cat#M0525S XhoI New England Biolabs Cat#R0146S NdeI New England Biolabs Cat#R0111S Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) New England Biolabs Cat#E0552S ZymoPURE Plasmid MiniPrep Kit Zymo Research Corporation Cat#50-125-1483 Zymoclean Gel DNA Recovery Kit Zymo Research Corporation Cat#11-301 Protein purification and validation Source Identifier Lysozyme, chicken egg white Sigma-Aldrich Cat#L6876-5G Ribonuclease A, bovine pancreas, chromatographically purified Thermo Fisher Scientific Cat#J62232EX3 DNase I Thermo Fisher Scientific Cat#EN0521 Fisher Science Education Seamless Cellulose Dialysis Tubing (14,000 MWCO) Thermo Fisher Scientific Cat#S25645H 12% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Laboratories Cat#4568046 Precision Plus Protein Unstained Standards Bio-Rad Laboratories Cat#1610363 Water Purification System Source Identifier Milli-Q Advantage A10 with BioPak Polisher, 18.2 MΩ·cm Millipore Sigma Cat# Z00Q0V0WW Proteases Source Identifier TEV Protease (TEVP) New England Biolabs Cat#P8112S Thrombin, Bovine, High Activity Calbiochem/Sigma-Aldrich Cat#604980-100U Factor Xa Protease New England Biolabs Cat#P8010S Enterokinase, light chain New England Biolabs Cat#P8070S Oligonucleotides Source Identifier ssDNA Integrated DNA Technologies N/A Gene fragments Source Identifier dsDNA Twist Bioscience N/A Recombinant DNA Source Identifier pGGAselect DNA New England Biolabs Component in Cat#E1602S and Cat#E1601S SI-3 Modified pET-22b(+) expression vector (SI section 4) This work - pET22b-Empty-cleavableELP (SI section 2) This work Addgene ID - 219401 pET22b-TEVP-cleavableELP This work Addgene ID - 219402 pET22b-FXa-cleavableELP This work Addgene ID - 219403 pET22b-EK-cleavableELP This work Addgene ID - 219404 pET22b-Thr-cleavableELP This work Addgene ID - 219405 pET22b-ELP[V]60 This work Addgene ID - 219406 SI-4 2.

    Staining:

    Article Title: Protease-Driven Phase Separation of Elastin-Like Polypeptides.
    Article Snippet: E. coli Transformation Kit and Buffer Set Zymo Research Corporation Cat#T3002 Cloning Source Identifier NEBridge Golden Gate Assembly Kit (BsmBI-v2) New England Biolabs Cat#E1602S NEBridge Golden Gate Assembly Kit (BsaI-HF v2) New England Biolabs Cat#E1601S Plasmid-Safe ATP-Dependent DNAse LGC Biosearch Technologies Cat#NC9046399 SacI-HF New England Biolabs Cat#R3156S HindIII-HF New England Biolabs Cat#R3104S T4 DNA Ligase New England Biolabs Cat#M0202L Quick CIP New England Biolabs Cat#M0525S XhoI New England Biolabs Cat#R0146S NdeI New England Biolabs Cat#R0111S Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) New England Biolabs Cat#E0552S ZymoPURE Plasmid MiniPrep Kit Zymo Research Corporation Cat#50-125-1483 Zymoclean Gel DNA Recovery Kit Zymo Research Corporation Cat#11-301 Protein purification and validation Source Identifier Lysozyme, chicken egg white Sigma-Aldrich Cat#L6876-5G Ribonuclease A, bovine pancreas, chromatographically purified Thermo Fisher Scientific Cat#J62232EX3 DNase I Thermo Fisher Scientific Cat#EN0521 Fisher Science Education Seamless Cellulose Dialysis Tubing (14,000 MWCO) Thermo Fisher Scientific Cat#S25645H 12% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Laboratories Cat#4568046 Precision Plus Protein Unstained Standards Bio-Rad Laboratories Cat#1610363 Water Purification System Source Identifier Milli-Q Advantage A10 with BioPak Polisher, 18.2 MΩ·cm Millipore Sigma Cat# Z00Q0V0WW Proteases Source Identifier TEV Protease (TEVP) New England Biolabs Cat#P8112S Thrombin, Bovine, High Activity Calbiochem/Sigma-Aldrich Cat#604980-100U Factor Xa Protease New England Biolabs Cat#P8010S Enterokinase, light chain New England Biolabs Cat#P8070S Oligonucleotides Source Identifier ssDNA Integrated DNA Technologies N/A Gene fragments Source Identifier dsDNA Twist Bioscience N/A Recombinant DNA Source Identifier pGGAselect DNA New England Biolabs Component in Cat#E1602S and Cat#E1601S SI-3 Modified pET-22b(+) expression vector (SI section 4) This work - pET22b-Empty-cleavableELP (SI section 2) This work Addgene ID - 219401 pET22b-TEVP-cleavableELP This work Addgene ID - 219402 pET22b-FXa-cleavableELP This work Addgene ID - 219403 pET22b-EK-cleavableELP This work Addgene ID - 219404 pET22b-Thr-cleavableELP This work Addgene ID - 219405 pET22b-ELP[V]60 This work Addgene ID - 219406 SI-4 2.

    Activity Assay:

    Article Title: Protease-Driven Phase Separation of Elastin-Like Polypeptides.
    Article Snippet: E. coli Transformation Kit and Buffer Set Zymo Research Corporation Cat#T3002 Cloning Source Identifier NEBridge Golden Gate Assembly Kit (BsmBI-v2) New England Biolabs Cat#E1602S NEBridge Golden Gate Assembly Kit (BsaI-HF v2) New England Biolabs Cat#E1601S Plasmid-Safe ATP-Dependent DNAse LGC Biosearch Technologies Cat#NC9046399 SacI-HF New England Biolabs Cat#R3156S HindIII-HF New England Biolabs Cat#R3104S T4 DNA Ligase New England Biolabs Cat#M0202L Quick CIP New England Biolabs Cat#M0525S XhoI New England Biolabs Cat#R0146S NdeI New England Biolabs Cat#R0111S Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) New England Biolabs Cat#E0552S ZymoPURE Plasmid MiniPrep Kit Zymo Research Corporation Cat#50-125-1483 Zymoclean Gel DNA Recovery Kit Zymo Research Corporation Cat#11-301 Protein purification and validation Source Identifier Lysozyme, chicken egg white Sigma-Aldrich Cat#L6876-5G Ribonuclease A, bovine pancreas, chromatographically purified Thermo Fisher Scientific Cat#J62232EX3 DNase I Thermo Fisher Scientific Cat#EN0521 Fisher Science Education Seamless Cellulose Dialysis Tubing (14,000 MWCO) Thermo Fisher Scientific Cat#S25645H 12% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Laboratories Cat#4568046 Precision Plus Protein Unstained Standards Bio-Rad Laboratories Cat#1610363 Water Purification System Source Identifier Milli-Q Advantage A10 with BioPak Polisher, 18.2 MΩ·cm Millipore Sigma Cat# Z00Q0V0WW Proteases Source Identifier TEV Protease (TEVP) New England Biolabs Cat#P8112S Thrombin, Bovine, High Activity Calbiochem/Sigma-Aldrich Cat#604980-100U Factor Xa Protease New England Biolabs Cat#P8010S Enterokinase, light chain New England Biolabs Cat#P8070S Oligonucleotides Source Identifier ssDNA Integrated DNA Technologies N/A Gene fragments Source Identifier dsDNA Twist Bioscience N/A Recombinant DNA Source Identifier pGGAselect DNA New England Biolabs Component in Cat#E1602S and Cat#E1601S SI-3 Modified pET-22b(+) expression vector (SI section 4) This work - pET22b-Empty-cleavableELP (SI section 2) This work Addgene ID - 219401 pET22b-TEVP-cleavableELP This work Addgene ID - 219402 pET22b-FXa-cleavableELP This work Addgene ID - 219403 pET22b-EK-cleavableELP This work Addgene ID - 219404 pET22b-Thr-cleavableELP This work Addgene ID - 219405 pET22b-ELP[V]60 This work Addgene ID - 219406 SI-4 2.

    Recombinant:

    Article Title: Protease-Driven Phase Separation of Elastin-Like Polypeptides.
    Article Snippet: E. coli Transformation Kit and Buffer Set Zymo Research Corporation Cat#T3002 Cloning Source Identifier NEBridge Golden Gate Assembly Kit (BsmBI-v2) New England Biolabs Cat#E1602S NEBridge Golden Gate Assembly Kit (BsaI-HF v2) New England Biolabs Cat#E1601S Plasmid-Safe ATP-Dependent DNAse LGC Biosearch Technologies Cat#NC9046399 SacI-HF New England Biolabs Cat#R3156S HindIII-HF New England Biolabs Cat#R3104S T4 DNA Ligase New England Biolabs Cat#M0202L Quick CIP New England Biolabs Cat#M0525S XhoI New England Biolabs Cat#R0146S NdeI New England Biolabs Cat#R0111S Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) New England Biolabs Cat#E0552S ZymoPURE Plasmid MiniPrep Kit Zymo Research Corporation Cat#50-125-1483 Zymoclean Gel DNA Recovery Kit Zymo Research Corporation Cat#11-301 Protein purification and validation Source Identifier Lysozyme, chicken egg white Sigma-Aldrich Cat#L6876-5G Ribonuclease A, bovine pancreas, chromatographically purified Thermo Fisher Scientific Cat#J62232EX3 DNase I Thermo Fisher Scientific Cat#EN0521 Fisher Science Education Seamless Cellulose Dialysis Tubing (14,000 MWCO) Thermo Fisher Scientific Cat#S25645H 12% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Laboratories Cat#4568046 Precision Plus Protein Unstained Standards Bio-Rad Laboratories Cat#1610363 Water Purification System Source Identifier Milli-Q Advantage A10 with BioPak Polisher, 18.2 MΩ·cm Millipore Sigma Cat# Z00Q0V0WW Proteases Source Identifier TEV Protease (TEVP) New England Biolabs Cat#P8112S Thrombin, Bovine, High Activity Calbiochem/Sigma-Aldrich Cat#604980-100U Factor Xa Protease New England Biolabs Cat#P8010S Enterokinase, light chain New England Biolabs Cat#P8070S Oligonucleotides Source Identifier ssDNA Integrated DNA Technologies N/A Gene fragments Source Identifier dsDNA Twist Bioscience N/A Recombinant DNA Source Identifier pGGAselect DNA New England Biolabs Component in Cat#E1602S and Cat#E1601S SI-3 Modified pET-22b(+) expression vector (SI section 4) This work - pET22b-Empty-cleavableELP (SI section 2) This work Addgene ID - 219401 pET22b-TEVP-cleavableELP This work Addgene ID - 219402 pET22b-FXa-cleavableELP This work Addgene ID - 219403 pET22b-EK-cleavableELP This work Addgene ID - 219404 pET22b-Thr-cleavableELP This work Addgene ID - 219405 pET22b-ELP[V]60 This work Addgene ID - 219406 SI-4 2.

    Modification:

    Article Title: Protease-Driven Phase Separation of Elastin-Like Polypeptides.
    Article Snippet: E. coli Transformation Kit and Buffer Set Zymo Research Corporation Cat#T3002 Cloning Source Identifier NEBridge Golden Gate Assembly Kit (BsmBI-v2) New England Biolabs Cat#E1602S NEBridge Golden Gate Assembly Kit (BsaI-HF v2) New England Biolabs Cat#E1601S Plasmid-Safe ATP-Dependent DNAse LGC Biosearch Technologies Cat#NC9046399 SacI-HF New England Biolabs Cat#R3156S HindIII-HF New England Biolabs Cat#R3104S T4 DNA Ligase New England Biolabs Cat#M0202L Quick CIP New England Biolabs Cat#M0525S XhoI New England Biolabs Cat#R0146S NdeI New England Biolabs Cat#R0111S Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) New England Biolabs Cat#E0552S ZymoPURE Plasmid MiniPrep Kit Zymo Research Corporation Cat#50-125-1483 Zymoclean Gel DNA Recovery Kit Zymo Research Corporation Cat#11-301 Protein purification and validation Source Identifier Lysozyme, chicken egg white Sigma-Aldrich Cat#L6876-5G Ribonuclease A, bovine pancreas, chromatographically purified Thermo Fisher Scientific Cat#J62232EX3 DNase I Thermo Fisher Scientific Cat#EN0521 Fisher Science Education Seamless Cellulose Dialysis Tubing (14,000 MWCO) Thermo Fisher Scientific Cat#S25645H 12% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Laboratories Cat#4568046 Precision Plus Protein Unstained Standards Bio-Rad Laboratories Cat#1610363 Water Purification System Source Identifier Milli-Q Advantage A10 with BioPak Polisher, 18.2 MΩ·cm Millipore Sigma Cat# Z00Q0V0WW Proteases Source Identifier TEV Protease (TEVP) New England Biolabs Cat#P8112S Thrombin, Bovine, High Activity Calbiochem/Sigma-Aldrich Cat#604980-100U Factor Xa Protease New England Biolabs Cat#P8010S Enterokinase, light chain New England Biolabs Cat#P8070S Oligonucleotides Source Identifier ssDNA Integrated DNA Technologies N/A Gene fragments Source Identifier dsDNA Twist Bioscience N/A Recombinant DNA Source Identifier pGGAselect DNA New England Biolabs Component in Cat#E1602S and Cat#E1601S SI-3 Modified pET-22b(+) expression vector (SI section 4) This work - pET22b-Empty-cleavableELP (SI section 2) This work Addgene ID - 219401 pET22b-TEVP-cleavableELP This work Addgene ID - 219402 pET22b-FXa-cleavableELP This work Addgene ID - 219403 pET22b-EK-cleavableELP This work Addgene ID - 219404 pET22b-Thr-cleavableELP This work Addgene ID - 219405 pET22b-ELP[V]60 This work Addgene ID - 219406 SI-4 2.

    Expressing:

    Article Title: Protease-Driven Phase Separation of Elastin-Like Polypeptides.
    Article Snippet: E. coli Transformation Kit and Buffer Set Zymo Research Corporation Cat#T3002 Cloning Source Identifier NEBridge Golden Gate Assembly Kit (BsmBI-v2) New England Biolabs Cat#E1602S NEBridge Golden Gate Assembly Kit (BsaI-HF v2) New England Biolabs Cat#E1601S Plasmid-Safe ATP-Dependent DNAse LGC Biosearch Technologies Cat#NC9046399 SacI-HF New England Biolabs Cat#R3156S HindIII-HF New England Biolabs Cat#R3104S T4 DNA Ligase New England Biolabs Cat#M0202L Quick CIP New England Biolabs Cat#M0525S XhoI New England Biolabs Cat#R0146S NdeI New England Biolabs Cat#R0111S Q5 Site-Directed Mutagenesis Kit (Without Competent Cells) New England Biolabs Cat#E0552S ZymoPURE Plasmid MiniPrep Kit Zymo Research Corporation Cat#50-125-1483 Zymoclean Gel DNA Recovery Kit Zymo Research Corporation Cat#11-301 Protein purification and validation Source Identifier Lysozyme, chicken egg white Sigma-Aldrich Cat#L6876-5G Ribonuclease A, bovine pancreas, chromatographically purified Thermo Fisher Scientific Cat#J62232EX3 DNase I Thermo Fisher Scientific Cat#EN0521 Fisher Science Education Seamless Cellulose Dialysis Tubing (14,000 MWCO) Thermo Fisher Scientific Cat#S25645H 12% Mini-PROTEAN TGX Stain-Free Protein Gels Bio-Rad Laboratories Cat#4568046 Precision Plus Protein Unstained Standards Bio-Rad Laboratories Cat#1610363 Water Purification System Source Identifier Milli-Q Advantage A10 with BioPak Polisher, 18.2 MΩ·cm Millipore Sigma Cat# Z00Q0V0WW Proteases Source Identifier TEV Protease (TEVP) New England Biolabs Cat#P8112S Thrombin, Bovine, High Activity Calbiochem/Sigma-Aldrich Cat#604980-100U Factor Xa Protease New England Biolabs Cat#P8010S Enterokinase, light chain New England Biolabs Cat#P8070S Oligonucleotides Source Identifier ssDNA Integrated DNA Technologies N/A Gene fragments Source Identifier dsDNA Twist Bioscience N/A Recombinant DNA Source Identifier pGGAselect DNA New England Biolabs Component in Cat#E1602S and Cat#E1601S SI-3 Modified pET-22b(+) expression vector (SI section 4) This work - pET22b-Empty-cleavableELP (SI section 2) This work Addgene ID - 219401 pET22b-TEVP-cleavableELP This work Addgene ID - 219402 pET22b-FXa-cleavableELP This work Addgene ID - 219403 pET22b-EK-cleavableELP This work Addgene ID - 219404 pET22b-Thr-cleavableELP This work Addgene ID - 219405 pET22b-ELP[V]60 This work Addgene ID - 219406 SI-4 2.



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